TankblotFor gentle blotting of large and temperature-sensitive proteins
- Simultaneous blotting of up to four 10 x 10 cm gels
- Integrated cooling-jacket for effective cooling
- Homogeneous buffer temperature
Electro-blotting is an important electrophoresis method to transfer mainly proteins from polyacrylamide gels on to nitrocellulose or other carrier membranes. During tank blotting proteins are transferred in a vertical buffer tank between coiled platinum wire electrodes arranged on two sides. For this technique, the gel and blotting membrane are clamped in a frame between filter paper and fiber pads.
In the Tankblot a cooling jacket is integrated into the sidewalls. This results in convective chilling by circulation: the temperature is distributed more homogeneously than with other designs (± 0.5ºC at 6ºC buffer temperature). This special design also prevents formation of ion gradients.
The Tankblot is recommended particularly for blotting of large molecules (> 100 resp. 200 kDa, depending on the proteins' composition) or of proteins which are difficult to transfer. The Tankblot is the instrument of choice also for native (temperature-sensitive) enzymes.
By selecting an appropriate buffer, transfer can be fast (between 30 min. and 4 h) or, under exceptionally gentle conditions, overnight (ca. 12 h). Thanks to the buffer volume of 1.2 liters transfer times of more than 24 h can be realised, e. g. for gentle blotting of very big proteins.
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Core compentencies
Electrophoresis
