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To find the optimal annealing temperature of new primers pairs the TProfessional TRIO Thermocycler offers the new Temperature Optimisation Step function. Temperature Optimisation Steps, by making use of the three blocks, provide different annealing temperatures in a gradient-like fashion. In the corresponding screen just enter an annealing temperature for the block in the middle and an increment, defining the temperature difference for the first and third block. For maximum ease of use programs containing Temperature Optimisation Steps automatically start on all three blocks, there is no need to start and stop the blocks individually.