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Custom Oligonucleotide Synthesis

  • Oligos are delivered in solution, ready to use with a defined concentration of 100pmol/µl
  • Broad range of fluorescent labels
  • RNA synthesis

Biometra oligonucleotides are available in three different purification grades

Molecular Biology grade purification

Salts, base protecting groups and short failure sequences up to 6 bases are removed efficiently. The final yield of full length product depends on chain length and coupling efficiancy. The synthesis of a typical 20mer with an average coupling yield of 98.5 % yields 70 % of full length product. This purification is recommended for standard molecular biology applications such as PCR, sequencing (oligonucleotides up to 30 bases).

PCR grade purification (HPLC)

In addition to the removal of salts and base protecting groups, the trityl-on HPLC step efficiently removes trityl-off failure sequences. This purification is recommended for all applications where full-length primers or high reproducibility are essential, including PCR, cloning, Mutagenesis, modified oligonucleotides and all oligonucleotides longer than 40 bases.

Cell culture grade purification

This unique in-house development is a multi-step purification method for all oligonucleotides used in cell culture. It consists of two HPLC purification steps and one sterile filtration step  

Biometra offers a broad range of DNA and RNA synthesis and fluorescent dyes. For more information please contact info@biometra.com.