Traditional method for transfer of nucleic acids
- Membrane size up to 20 x 25 cm
- Overnight transfer
- Easy to use
Method for transfer of nucleic acids (especially RNA) from agarose gels onto a membrane. This procedure usually takes up to 12 hours (over night) to complete.
Easy to set up assembly that works without power or vacuum source.
This method is based on the movement of buffer from a reservoir through the gel and the blotting membrane to a stack of dry blotting paper by capillary force. The molecules are carried to the blotting membrane on which they are adsorbed.